EFFECTIVITY OF 50% ETHANOL EXTRACT OF CAMELLIA SINENSIS TEA LEAVES AS HAND ANTISEPTIC

Authors

  • Monica Oktaviana Fakultas Kedokteran dan Ilmu Kesehatan Unika Atma Jaya
  • Johannes C Prihadi Departemen Ilmu Bedah, Fakultas Kedokteran dan Ilmu Kesehatan Unika Atma Jaya
  • Lucky H. Moehario Departemen Mikrobiologi, Fakultas Kedokteran dan Ilmu Kesehatan Unika Atma Jaya

DOI:

https://doi.org/10.25170/djm.v20i1.1835

Keywords:

antiseptic, Camellia sinensis, hand washing

Abstract

Introduction: : Practicing hand hygiene is a way to control an infection. The recent COVID-19 pandemic, hand hygiene has become the necessity. Using antiseptic which contain chemical active ingredients causes skin problem. Camellia sinensis is a natural ingredient which its antimicrobial properties is due to the presence of catechin. The study was aimed to determine the effectivity of ethanol extract of Camellia sinensis tea leaves as hand antiseptic.

Methods: This research was a comparative experimental analytic to 32 respondents conducted in the Microbiology Laboraroty of Faculty Medicine and Health Sciences–Atma Jaya Jakarta Catholic University of Indonesia. Sampling was carried out by taking bacterial swabs on the right palms. Followed by dilution using serial dilution method and inoculated onto nutrient agar using the spread plate technic. After incubation at 37oC overnight the bacterial colonies were calculated using colony counter within a range of 30-300 colonies per plate. Wilcoxon and Mann-Whitney was used for data analysis.

Results: The total number of bacterial colonies after sanitizing using 50% ethanol extract of Camellia sinensis tea leaves was reduced by 55.04% (p=0.003). Meanwhile, 70% alcohol curb the bacterial colonies by 76.84% (p=0.000). The comparison of the effectivity of 50% ethanol extract of Camellia sinensistea leaves versus 70%  alcohol as hand antiseptic was insignificance (p=0.300).

Conclusion: 50% ethanol extract of Camellia sinensis tea leaves showed activity against hand’s microorganisms. However, the percentages of Camellia sinensis extract might be increased so as to achieve the effectivity of 70% alcohol.

Downloads

Download data is not yet available.

References

Jindal R, Pandhi D. Hand hygiene practices and risk and prevention of hand eczema during the COVID19 pandemic. Indian Dermatol Online J. 2020.

Kementerian Kesehatan Republik Indonesia. Peraturan Menteri Kesehatan Republik Indonesia. PPI Fasilitas Pelayanan Kesehatan. 2017

Beiu C, Mihai M, Popa L, et al. Frequent hand washing for COVID-19 prevention can cause hand
dermatitis: Management tips. Cureus.2020

Larson E, Girard R, Pessoa-Silva CL, Boyce J, Donaldson L, Pittet D. Skin reactions related to hand hygiene and selection of hand hygiene products. Am J Infect Control. 2014; 34(10):627–635.

Wolverton W, Gada S. Systemic contact dermatitis to ethanol. Am J Clin Dermatol. 2013;1(2):195.

Yang Y, Zhang T. Antimicrobial activities of tea polyphenol on phytopathogens: A review. Molecules. 2019;24(4).

Al-Thalib H, Al-Khateeb A, Murugaiah C. Antimicro-bial effect of Camellia sinensis on the skin micro-biota. Malays J Microbiol. 2016;12(6): 423-427.

Reygaert WC. Green tea catechins: Their use in treating and preventing infectious disease. BioMed Res Int. 2018.

Zhang L-Q, Wei K, Cheng H, Wang L-Y, Zhang C-C. Accumulation of catechins and expression of catechin synthetic genes in Camellia sinensis at different developmental stages. Bot Stud. 2016;57(1):31.

Zeniusa P, Ramadhian R. Efektivitas ekstrak etanol teh hijau dalam menghambat pertumbuhan Eschericia coli [skripsi].Lampung: Fakultas Kedokteran, Universitas Lampung; 2017.

Dwipriastuti D, Putranto. Perbedaan efektivitas chlorhexidine glukonat 0,2% dengan teh hijau (Camellia sinensis) terhadap jumlah Porphyro-monas gingivalis. Odovtos Int J Dent Sc. 2017;4(1).

Maryam H. Aktivitas antimikroba ekstrak daun teh hijau (Camellia sinensis) dan Jati Belanda (Guazuma ulmifolia Lamk) terhadap mikroba patogen secara bioautography-TLC. AS-SYIFA Jurnal Farmasi. 2017;9(1):92-98.

Setiawan I, Saryantu D. Pemanfaatan ekstrak daun teh (Camellia sinensis L) dari perkebunan kemuning kab. karang anyar dalam pembuatan sabun padat transparan uji aktivitas antibakteri pada Staphylococcus aureus. Jurnal Riset Kefarmasian Indonesia. 2019; 1(1).

Jensen D, Macinga D, Shumaker D. Quantifying the effects of water temperature, soap volume, lather time and antimicrobial soap as variabels in the removal of Escherichia coli ATCC 11229 from hand. J Food Prot. 2017; 80(6).

Bay L, Barnes CJ, Fritz BG, Thorsen J, Odgaard A, Bjarnsholt T, et al. Universal dermal microbiome in human skin. mBio. 2020; 11(1).

Purwanti S, Arundina A, Yanti SN. Perilaku mencuci tangan terhadap angka koloni kuman pada penjamah makanan di kantin Universitas Tanjungpura. Jurnal Vokasi Kesehatan. 2015;1(2): 64-69.

Norfai A. Efektivitas penggunaan sabun dalam mencuci tangan terhadap jumlah kuman. J Publikasi Kesehat Masy Indones 2018; 5(2).

Ho HJ, Poh B-F, Choudhury S, Krishnan P, Ang B, Chow A. Alcohol handrubbing and chlorhexidine handwashing are equally effective in removing Methicillin-Resistant Staphylococcus aureus from health care workers’ hands: A randomized controlled trial. Am J Infect Control. 2015; 43(11):1246–8.

Gold NA, Avva U. Alcohol sanitizer. StatPearls Publishing; 2019.

Sari AW. Karakteristik ekstrak etanolik daun teh hijau (Camellia sinensis L.)[skripsi]. Yogyakarta: Fakultas Farmasi, Universitas Sanata Dharma; 2010.

Roy N, Mandal S, Mahanti B, Dasgupta S. Antimicrobial activity of green tea: A comparative study with different green tea extract; PharmaTutor; 2018; 6(1); 23-29.

Lingga AR, Pato U, Rossi E. Uji Antibakteri ekstrak batang Kecombrang (Nicolaia speciosa Horan) terhadap Staphylococcus aureus dan Escherichia coli. JOM Faperrta. 2015;2(2).

Downloads

Published

2021-05-31

How to Cite

1.
Oktaviana M, Prihadi JC, Moehario LH. EFFECTIVITY OF 50% ETHANOL EXTRACT OF CAMELLIA SINENSIS TEA LEAVES AS HAND ANTISEPTIC. DJM [Internet]. 2021 May 31 [cited 2024 Mar. 28];20(1):9-17. Available from: https://ejournal.atmajaya.ac.id/index.php/damianus/article/view/1835
Abstract views: 288 | PDF downloads: 268