AKTIVITAS ANTIOKSIDAN DAN ANTI HIPERKOLESTEROLEMIA EKSTRAK UMBI BAWANG DAYAK (Eleutherine bulbosa [Mill.] Urb)

  • Yani Andryani Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Kristen Krida Wacana, Jakarta, Indonesia
  • Anna Maria Dewajanti Departemen Biokimia, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Kristen Krida Wacana, Jakarta, Indonesia
  • Adelina Simamora Departemen Biokimia, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Kristen Krida Wacana, Jakarta, Indonesia
Keywords: Dayak onion bulbs, DPPH, flavonoid content, hypercholesterolemia

Abstract

Introduction: High cholesterol levels can build up plaque inside the lining of the artery wall leading to coronary heart disease. Dayak onion bulbs (Eleutherine bulbosa (Mill.) Urb) contain flavonoids that can act as antioxidants and are believed to reduce blood cholesterol level. This study investigated the flavonoid content and antioxidant activity of Dayak onion tuber extract, and their effect on total blood cholesterol levels.

Methods: Dayak onion bulbs were extracted by maceration (ethanol 70%). Total flavonoid was determined by a colorimetric method using AlCl3. Antioxidant activity was determined based on DPPH radical scavenging activity. Anti-hypercholesterolemia effect was studied in vitro. Thirty-six white male rats (Rattus novergicus) were divided into 6 groups: healthy rats, hyper-cholesterol rats, hyper-cholesterol rats treated with simvastatin (18 mg/bw) and rutin (75 mg/bw) as positive controls, hyper-cholesterol rats treated with dayak union bulbs extracts (75 and 150 mg/bw). Measurement of total cholesterol levels was performed using CHOD-PAP method. Data were analyzed using paired t-test and one-way anova.

Results: Flavonoids were present in onion bulb extract with a concentration of 1.7 mg/g dried biomass. The antioxidant activity of the extract (IC50 0.37 mg/mL) was weaker than that of rutin (IC50 15.58 µg/mL). The addition of dayak onion bulbs extract dose of 150 mg/kg bw was the best dose in reducing total blood cholesterol levels on the 14th day, p = 0,001.

Conclusion: dayak onion bulbs can be further investigated for application as anti hypercholesterolemia agent.

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Author Biographies

Yani Andryani, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Kristen Krida Wacana, Jakarta, Indonesia

Yani Andryani is a medical student at the Faculty of Medicine Krida Wacana Christian University.

Adelina Simamora, Departemen Biokimia, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Kristen Krida Wacana, Jakarta, Indonesia

Adelina Simamora, MS, MSc (Pharm) is a researcher in the center for enzymes in health and diseases studies and a lecturer in Biochemistry at the Faculty of Medicine Krida Wacana Christian University. At present, her research focus on the bioactive potential of medicinal plants, with special interest in various enzyme inhibition properties. Currently, her work involves various Indonesian medicinal plants and inhibition properties on α-glucosidase, xanthine oxidase, and lipase. Her interest also spans to transition and rare earth metals and their application as sensor in biological fluid.

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Published
2022-04-30
How to Cite
1.
Andryani Y, Dewajanti A, Simamora A. AKTIVITAS ANTIOKSIDAN DAN ANTI HIPERKOLESTEROLEMIA EKSTRAK UMBI BAWANG DAYAK (Eleutherine bulbosa [Mill.] Urb) [Internet]. Damianus Journal of Medicine [Internet]. 30Apr.2022 [cited 7Oct.2022];21(1):1-4. Available from: https://ejournal.atmajaya.ac.id/index.php/damianus/article/view/3362
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