Pengaruh Variasi Ukuran Partikel pada Ekstraksi Biji Alpukat (Persea americana Mill.) Terhadap Penurunan Kadar Trigliserida Pada Tikus Hiperlipidemia

Abstrak

Background: Avocado seed (Persea americana Mill.) which is usually thrown away after avocado fruit consumption, contains active compounds that can lower blood triglyceride level. Utilization of avocado seed as a medication to lower triglyceride has potentials that still needs to be discovered before it can be used as an alternative herbal medicine.

Objectives: The purpose of this research is to find out the effect of avocado seed’s particle size variation during extraction on the extract effectiveness in lowering blood triglyceride level.

Methods: This is an experimental research which is done on Sprague Dawley rats. The rats are propylthiouracil (PTU) for 14 days to reach hyperlipidemic state. The hyperlipidemic rats are divided into 2 groups of intervention. The first group receives avocado seed extract which was extracted from avocado seeds that had been milled for 15 minutes. The second group receives avocado seed extract which was extracted from avocado seeds that had been milled for 30 minutes. Particle size is determined by the average diameter of 10 random avocado seed particle in each group. The avocado seed particle is extracted using remaceration method. Avocado seed extract is given for 14 days with a daily dose of 250 mg/kgBW. Triglyceride level in the rats’ blood is measured after PTU has been given for 14 days and is measured again after 7 days, and 14 days of avocodo seed extract intervention. Unpaired T-test and Paired T-test are used to analyze the data.

Results: The average particle size of avocado seed in the second group avocado seed’s extraction  the second group is 13.50 µm. Avocado seed extract lowers blood triglyceride level on hyperlipidemic rats from day 0-7, day 0-14 and day 7-14 in the first group (p<0.05). The findings in the second group are similar (p<0.05), except for day 7-14 (p>0.05). There is no significant difference in the decrease of blood triglyceride level between the first group and the second group (p>0.05).

Conclusions: Avocado seed extract can lower the level of blood triglyceride in rats. The variation of avocado seed’s particle size on its extraction has no effect on the decrease of blood triglyceride.