Potensi daun jelatang (Laportea interrupta) sebagai penghambat pertumbuhan Trichopyton rubrum
DOI:
https://doi.org/10.25170/djm.v24i3.6926Keywords:
Trichophyton rubrum, Laportea interrupta, antifungi, konsentrasi hambat minimumAbstract
Pendahuluan: Dermatofitosis merupakan infeksi kulit menular yang disebabkan oleh jamur dermatofita, terutama Trichophyton rubrum. Jenis yang paling sering ditemukan di Indonesia adalah Tinea corporis dan Tinea cruris. Meningkatnya resistensi T. rubrum terhadap antijamur seperti terbinafine dan azole mendorong perlunya alternatif pengobatan, salah satunya tanaman herbal. Laportea interrupta (jelatang) merupakan tanaman tradisional yang diketahui memiliki senyawa aktif seperti flavonoid, alkaloid, dan saponin yang berpotensi sebagai antimikroba. Saat ini belum ada penelitian yang menguji aktivitas antifungi L. interrupta terhadap T. rubrum.
Metode: Metode pengujian antifungi menggunakan Broth Microdilution Method pada 96-well Microtiter Plate. Analisis statistic dilakukan menggunakan Welch Anova.
Hasil: Ekstrak etanol daun L. interrupta menunjukkan aktivitas antifungi terhadap T. rubrum, dengan konsentrasi 37.500–150.000 μg/ml mampu menghambat pertumbuhan jamur secara efektif dan sebanding dengan flukonazol. Sebaliknya, konsentrasi di bawah 18.750 μg/ml tidak menunjukkan efek penghambatan yang signifikan. Analisis statistik menggunakan One-way ANOVA menunjukkan perbedaan yang bermakna dari masing-masing kelompok perlakuan (p=0,000).
Simpulan: Ekstrak etanol daun L. interrupta efektif menghambat pertumbuhan T. rubrum pada konsentrasi 37.500–150.000 μg/ml, dengan Konsentrasi Hambat Minimum (KHM) sebesar 37.500 μg/ml
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References
1. Kimberly BT, Rini CS. Effectiveness test of okra fruit (Abelmoschus esculentus) extract on the growth of Trichophyton rubrum. Medicra (J Med Lab Sci/Technol). 2022;5(2):86–90.
2. Graceciela YE, Anggraini DI, Himayani R, Sibero HT. Hubungan usia, jenis kelamin, dan pekerjaan dengan kejadian dermatofitosis di Rumah Sakit Umum Daerah Dr. H. Abdul Moeloek Provinsi Lampung periode 2017–2021. Medula. 2024;14(6): 1036–45.
3. Natalia, Rosnita S, Ian K. Perbedaan jumlah koloni jamur Trichophyton rubrum pada media SDA. J Penelitian Sains. 2021;21(3):163–7.
4. Sacheli R, Hayette MP. Antifungal resistance in dermatophytes: Genetic considerations, clinical presentations and alternative therapies. J Fungi (Basel). 2021 Nov 18;7(11):983.
5. Monod M, Feuermann M, Salamin K, Fratti M, Makino M, Alshahni MM, et al. Trichophyton rubrum azole resistance mediated by a new ABC transporter, TruMDR3. Med Mycol. 2019;63(11):1–19.
6. Nirosa GD, Puspitasari P. Pengaruh ekstrak daun kedondong (Spondias dulcis) terhadap pertumbuhan Trichophyton rubrum secara in vitro. Medicra (J Med Lab Sci/Technol). 2019;2(2):68–73.
7. Bitencourt TA, Macedo C, Franco ME, Assis AF, Komoto TT, Stehling EG, et al. Transcription profile of Trichophyton rubrum conidia grown on keratin reveals the induction of an adhesin-like protein gene with a tandem repeat pattern. BMC Genomics. 2016 Mar 18;17:249.
8. Chinnapun D. Virulence factors involved in pathogenicity of dermatophytes. Walailak J Sci Technol. 2015;12(7):573–80.
9. Elavarashi E, Kindo AJ, Rangarajan S. Enzymatic and non-enzymatic virulence activities of dermatophytes on solid media. J Clin Diagn Res. 2017;11(2):DC23–5.
10. Blechert O, Zheng H, Zang X, Wang Q, Liu W. Influence of the cultivation medium and pH on the pigmentation of Trichophyton rubrum. PLoS One. 2019;14(9):1–12.
11. Achterman RR, White TC. Dermatophyte virulence factors: Identifying and analyzing genes that may contribute to chronic or acute skin infections. Int J Microbiol. 2012;2012.
12. Chang W, Liu J, Zhang M, Shi H, Zheng S, Jin X, et al. Efflux pump-mediated resistance to antifungal compounds can be prevented by conjugation with triphenylphosphonium cation. Nat Commun. 2018 Nov 30;9(1):5102.
13. Corsello A, Marangoni MB, Macchi M, Cozzi L, Agostoni C, Milani GP, et al. Febrile seizures: A systematic review of different guidelines. Pediatr Neurol [Internet]. 2024;155:141–8.
14. Simaremare ES, Ruban A, Runtuboi DYP. Aktivitas antibakteri ekstrak etanol daun gatal (Laportea aestuans (L.) Chew). J Biol Papua. 2018;9(1):1–7.
15. Cahyaningrum PL, Widyantari AAASS, Artini NPR. Skrining fitokimia ekstrak etanol daun jelatang ayam (Laportea interrupta (L.) Chew). Widya Kesehatan. 2022;4(1):15–23.
16. Pertiwi KK. Aktivitas antibakteri herba daun gatal (Laportea interrupta L. Chew) terhadap Staphylo-coccus aureus dan Escherichia coli. J-HESTECH. 2019;2(1):43.
17. Prithviraj Karak. Biological activities of flavonoids: An overview. Int J Pharm Sci Res. 2019;10(4): 1567–74.
18. Pangesty V, Kurniati ID, Ratnaningrum K. Effectiveness of bitter melon (Momordica charantia L.) extract on growth of Trichophyton rubrum: In vitro study. Magna Medica Berk Ilm Kedokteran dan Kesehatan. 2023;10(1):19.
19. Martins MP, Silva LG, Rossi A, Sanches PR, Souza LDR, Martinez-Rossi NM. Global analysis of cell wall genes revealed putative virulence factors in the dermatophyte Trichophyton rubrum. Front Microbiol. 2019;10(September):1–11.
20. Kar B, Patel P, Free SJ. Trichophyton rubrum LysM proteins bind to fungal cell wall chitin and to the N-linked oligosaccharides present on human skin glycoproteins. PLoS One. 2019;14(4):1–19.
21. Ruiz-Herrera J, Ortiz-Castellanos L. Cell wall glucans of fungi: A review. Cell Surface. 2019; 5(March):100022
22. Rita WS, Suprapta DN, Swantara IMD, Sudana IM. Antifungal activity of phenolic compounds from Samanea saman leaves against stem rot disease on dragon fruits caused by Fusarium solani. KnE Life Sciences. 2022;2022:621–35.
23. Ríos-López AL, Muñiz-Bernal V, Dávila-Aviña J, González GM, Treviño-Rangel RDJ, Becerril-García MA, et al. Antifungal and antivirulence activity of tannic acid against drug-resistant Candida species. Farmacia. 2024;72(4):946–56.
24. Hamzah H, Hertiani T, Pratiwi SUT, Nuryastuti T. The inhibition activity of tannin on the formation of mono-species and polymicrobial biofilm Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Trad Med J. 2019;24(2):110–8.
25. Rodrigues GCS, dos Santos Maia M, de Souza TA, de Oliveira Lima E, dos Santos LECG, Silva SL, et al. Antimicrobial potential of betulinic acid and investigation of the mechanism of action against nuclear and metabolic enzymes with molecular modeling. Pathogens. 2023;12(3).
26. Liu CY, Zhang L, Liu SX, Lu YF, Li C, Pei YH. A review of the fernane-type triterpenoids as anti-fungal drugs. Front Pharmacol. 2024;15(August):1-10.
27. Maisarah M, Chatri M, Advinda L. Karakteristik dan fungsi senyawa alkaloid sebagai antifungi pada tumbuhan. J Serambi Biol. 2023;8(2):231–6.
28. Octaviani M, Suryani W, Sandi NH. Aktivitas antijamur fraksi n-heksana kulit buah Citrus sinensis (L.) Osbeck. Jurnal Ilm Farm dan Farm Klin. 2024;2(1):59.
29. Hutomo S, Larope CG, Putri DU, Sooai CM, Kristiyanto HD. Antibacterial and anti-adherence effect of Laportea interrupta ethanolic extract on Streptococcus mutans biofilm. Dental J. 2025; 58(158):256–61.
30. Susilawati S, Anwar C, Saleh I, Salni S. Flavonoid as anti-Candida agents. Indones. J Fundamental Appl Chem. 2023;8(2):88–97.
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